微生物に於けるアシラーゼ能の研究 (第3報)

ε-N-Benzoyllysineのアシル誘導体に対する土壌細菌KT 84のアシラーゼ能

抄録

The acylase of soil bacteria KT 84 was extracted in a cell-free state and its cell-free acetone powder was prepared.
The bacterial mass, acetone-dried powder, cell-free extract, and cell-free acetone powder of KT 84 all effected asymmetric hydrolysis of α, ε-di-N-benzoyl- and α-N-acetyl-ε-N-benzoyl-DL-lysine affording ε-N-benzoyl-L-lysine and α, ε-di-N-benzoyl-D-lysine, and ε-N-benzoyl-L-lysine and α-N-acetyl-ε-N-bezoyl-D-lysine, respectively. The benzoyl derivative of ε-N-benzoyl-L-lysine was more easily hydrolysed than its acetyl derivative in this case.
Eleven kinds of α-N-acyl derivative of ε-N-benzoyl-DL-lysine were synthesized and hydrolytic action of cell-free extract of KT 84 was examined by ninhydrin colorimetry. The results showed that (1) m- and p-nitrobenzoyl, dichloroacetyl, and benzoyl derivatives were more easily hydrolyzed than other acyl derivatives; (2) m- and p-nitrobenzoyl derivatives were hydrolyzable but o-nitrobenzoyl was hardly hydrolyzed, while p-aminobenzoyl was far more resistant to hydrolysis than p-nitrobenzoyl derivative; (3) cinnamoyl and phenylacetyl derivatives are less hydrolyzable than benzoyl derivative; and that (4) chloroacetyl, acetyl, and formyl derivatives are much less hydrolyzable than dichloroacetyl derivative.