1959 年 79 巻 1 号 p. 32-34
Preparation and purification of callicrein from hog pancreas and its content in various organs were described in the preceding paper. It was now found that callicrein is selectively precipitated with acrinol (2-ethoxy-6, 9-diaminoacridinium lactate) and that this is an effective method for its purification. Callicrein of ca. 200U/mg. purity so obtained is positive to all coloration reaction for proteins in general and had absorption maximum in the region of 281 mμ in its ultraviolet spectrum.