ペンタシアノ鉄錯塩による有機化合物の呈色反応に関する研究 (第1報)

滬紙クロマトグラフィー

抄録

For separation of colored iron complex salts formed from pentacyano-iron complex, such as sodium nitroprusside and sodium pentacyanoammineferroate, and various organic compounds by paper chromatography, better result can be obtained by the use of a more polar solvent for development. Non-polar solvents cannot be used. Increased polarity of the developing solvent improves the reproducibility of Rf values but separation becomes poor. On the contrary, less polar solvent increases separation but reproducibility of Rf values becomes poor. The solvent systems having both suitable reproducibility of Rf values and separative ability were found to be the following: Sodium hydrogen carbonate (2g.)-ammonia water (40cc.)-acetone (60cc.); ammonium sulfate (10g.)-ammonia water (40cc.)-acetone (60cc.); sodium hydrogen carbonate (1.5g.)-ammonia water (30cc.)-methanol (70cc.), and ammonium sulfate (10g.)-ammonia water (30cc.)-methanol (70cc.).