スルファミン剤のN-グルクロナイドの研究 (第2報)

スルファピリジンの代謝産物としてのN-グルクロナイドについて

抄録

The increased amount of glucuronic acid found in the urine of a rabbit after administration of sulfapyridine was considered to be due to N-glucuronide formed as a metabolic product and the present series of experiments was carried out. The amount of various types of glucuronic acid in the urine of normal rabbit and that administered sulfapyridine was examined by the method of separatory determination of free acid, ester- and ether-type glucuronides, and amine N-glucuronide as described in the preceding paper. It was thereby found that majority of the increased excretion of total glucuronic acid was due to increased excretion of N-glucuronides, which comprised 35-41% of sulfapyridine administered. The lyophilized powder of the urine of a rabbit given sulfapyridine and synthesized sodium sulfapyridine N⁴-glucosiduronate was submitted to chromatography on filter paper buffered with the Universal Buffer Mixture. The Rf values on the chromatogram were found to agree at each pH and the N-glucuronide in the urine was thereby established.