Abstract
Pyruvic acid isonicotinoylhydrazone, the Schiff base of isonicotinic acid hydrazide (INAH) and pyruvic acid, was allowed to stand or heated in aqueous solution and the solution was submitted to paper partition chromatography, showing two spots with Rf values different from that of INAH. These spots appear more markedly on addition of Ca2+, Mg2+, and Ba2+. Development of these two spots by the two-dimensional paper chromatography resulted in further division of each into two spots. These two spots are considered to be of the same substance and are interconvertible.
