拡散因子の研究 (第1報)

ヒアルロニダーゼと拡散因子について

抄録

Intradermal diffusion of dyes by testicular hyaluronidase is considered to be due to hydrolysis of hyaluronic acid by enzymic action, resulting in the lowering of viscosity and increase in permeability. The hyaluronidase prepared from bovine testicle by ammonium sulfate purification was fractionated by zone electrophoresis with starch and spreading activity of each fraction was examined. It was thereby found that the fractions without any enzymic activity also had spreading activity. Aqueous solution of hyaluronidase, after having lost its enzymic activity by heat denaturation also showed some spreading activity.