粗睾丸ヒアルロニダーゼ標品中の蛋白分解酵素の存在とその除去
1960 年 80 巻 4 号 p. 552-554
詳細
1960 年 80 巻 4 号 p. 552-554
No report has been made on the protease contained in testicular tissue but its presence was detected in the crude hyaluronidase prepared by ammonium sulfate fractionation. This protease had optimal pH in the weakly acid range and was most stable at around pH 6. When left at pH 9 and 37°, in the presence of sodium chloride, the majority of protease activity could selectively be inactivated practically without any harm on the hyaluronidase activity.
