Steroids. XIV

Microbiological Oxidation of Reichstein's Substance S by Corticium sasakii. (1). Isolation of Hydrocortisone and 11-Epihydrocortisone

Abstract

The extract obtained by microbiological oxidation of Reichstein's substance S (I) by Corticium sasakii was submitted to paper partition chromatography with the solvent system of propyleneglycol/toluene·dioxane (7:3) and was separated into A, B, C, D, and E zones (Fig. 1). E zone was found to be the starting material (I), C zone, the hydrocortisone (II), and A zone was found to be a mixture of 11-epihydrocortisone (III) and a monohydroxylated substance S (IV), m. p. 233-236°, [α]_D+143°(EtOH), +127°(dioxane), U.V.λ_max^EtOH243.5mμ.