Abstract
Sulfisoxazole undergoes decomposition in vivo to form various kinds of product and a method for measuring blood level by isolation of unchanged Sulfisoxazole alone from such decomposition products was devised. For isolation of sulfisoxazole, liquid chromatography is employed, using powdered cellulose as the carrier and a mixture of butanol, propanol, and water in 2:1:1 ratio as the developing solvent. Determination of sulfisoxazole is made by colorimetry using p-dimethylaminobenzaldehyde and absorbancy is measured at 453mμ.
Since a fairly satisfactory result was obtained by this method, blood level was measured by this method after oral administration of sulfisoxazole and N1-acetylsulfisoxazole, and the result was compared with that from the widely used Bratton-Marshall's method.
