Abstract
Numerous reports have appeared on the determination of epirenamine and dl-norepirenamine. In order to establish a practicable colorimetric method of determination, examinations were made on various methods and it was found that colorimetric determination is possible by coloration with dimethyl-p-phenylenediamine as the reagent to effect indophenol reaction, extraction of the colored substance with isobutanol, and measurement of the optical density of the extract solution, this giving only a slight error. Examinations were also made on separatory determination of epirenamine and dl-norepirenamine, and epirenamine and phenylephrine. This separatory determination was found to be possible by the use of paper partition chromatography by the one-dimensional, ascending method, using a mixture of benzyl alcoholacetic acid-water (5:2:5) for epirenamine and dl-norepirenamine, and a mixture of butanol-acetic acid-water (5:2:5) for epirenamine and phenylephrine as the developing solvent.
