菌体外毒素のN-グルクロナイド生成に関する研究 その1

ブドウ菌毒素の溶血毒におよぼすグルクロン酸の影響

抄録

Effect of glucuronic acid on the hemolysin of extracellular toxin from staphylococcus in vitro was examined and its reaction mechanism was considered. Toxicity of this toxin weakened or disappeared easily on contact with glucuronic acid and the toxicity was not recovered on dialysis. Similar weakening and disappearance of toxicity was observed with glucuronamide, sodium galacturonate, and sodium pyruvate as with sodium glucuronate, but the effect of sodium gluconate and glucose was not clear. Since the toxicity of this toxin disappeared rapidly by treatment with 2, 4-dinitrofluorobenzene, free amino group in the toxin protein may be responsible for this toxicity. Therefore, weakening or disappearance of toxicity by glucuronic acid may be due to the reaction of glucuronic acid with the free amino group in the toxin protein, i.e. formation of N-glucuronide.