ステロイドホルモンの研究 (第1報)

テストステロンの呈色反応

抄録

Colorimetric determination of testosterone was devised by the use of blue coloration which testosterone and its esters show with ferrous ammonium sulfate. The reagent is prepared by dissolving 1g. of Mohr's salt in 20cc. of water, adding 1cc. of sulfuric acid, followed by 1cc. of 30% hydrogen peroxide, and boiling this mixture. The solution is cooled, diluted to 50cc. with water, and 3cc. of this solution is made up to 100cc. with sulfuric acid. A mixture of this reagent and testosterone or its esters are heated at 100° for 4 minutes in a water bath, cooled in an ice-water bath for 2 minutes, and 2cc, of water is added gently. This is shaken vigorously for 15 seconds by which testosterone shows characteristic blue color, The limit of detection by this coloration is 0.5γ of testosterone. When 20-2.5γ of testosterone was measured in a 10-mm. cell, linearity, parallelism, and fiducial limit were all found to be satisfactory.