Phosphorylcholineの簡易比色定量法

抄録

Phosphorylcholine forms a blue precipitate with Molybdenum Blue, obtained by reduction of phosphomolybdic acid with tin (II) chloride and colorimetric determination by this coloration reaction was devised. In this method, 0.4cc. of Molybdenum Blue solution (an equivolume mixture of 12% phosphomolybdic acid solution and 2% solution of SnCl₂⋅2H₂O in 2.5N hydrochloric acid) is added to 1cc. of the sample solution (0.1-0.4μmole), the mixture is cooled in ice for 2 hours, and the precipitate formed is collected by centrifugation. After washing the precipitate with 0.5% solution of SnCl₂⋅2H₂O in 0.1N hydrochloric acid, it is dissolved in 10cc. of equal mixture of acetone and 2.5N hydrochloric acid and coloni metric determination of this solution is carried out after 10-30 min. at 725mμ. Combination of this method with the cellulose column chromatography enabled rapid determination of phosphorylcholine in rat liver.