Abstract
Determination of bromovalerylurea in biological sample was devised. Plasma or urea is extracted with ether, the extract solution is washed with water, dried, and ether is evaporated. The residual extract is dissolved in dehyd. ethanol, 0.2M acetate buffer (pH 4.0) is added, and this solution is submitted to polarography between 0.0V and -1.6V. From its diffusion current, 5γ/cc. of bromovalerylurea can be determined. Recovery, 95-5%. This determination was carried out on the urea and blood of narcotism patients and observations were gained which would be of interest from the point of therapeutic diagnosis and forensic chemistry.
