Abstract
3-Methyl-2-benzothiazolinone hydrazone was found to undergo reaction with acrinol in the presence of ferric chloride to produce reddish violet color which has absorption maximum at 545mμ and this reaction was applied to quantitative determination of acrinol. Presence of 5-nitrofurfurylideneaminoguanidine, chloroprophenepyridamine maleate, and berberine hydrochloride does not interfere in this reaction. Procedure: One ml. of the test solution (containing 120μg./ml. of acrinol) is accurately measured into a 20ml. measuring flask, 1ml. of 0.5% 3-methyl-2-benzothiazolinone hydrazone solution is added, followed by 1ml. of 1.2% ferric chloride solution. The mixture is shaken well, warmed in a water bath of 60° for 10 minutes, and cooled. Ethanol (95%) is added to bring the whole volume to 20ml. Blank test is carried out with 1ml. of water in place of the test solution. The absorbance is measured at 545mμ, referred to this blank.
