有機分析 (第42報)
トリニトロベンゼンによる強心配糖体の比色定量
1963 年 83 巻 2 号 p. 143-147
詳細
1963 年 83 巻 2 号 p. 143-147
A sensitive colorimetric method was devised for determination of cardiac glycosides, oleandrin (I), digoxin (II), digitoxin (III), and ouabain (IV), with 1, 3, 5-trinitrobenzene as a color developing agent in sodium carbonate solution. The addition of sodium dihydrogenphosphate reduced the intensity of blank color.
The test solution was prepared by dissolving I, II, or III in dimethylformamide, and IV in water. To 1.0ml. of the test solution, 0.5ml. of 0.1% solution of trinitrobenzene and 0.5ml. of sodium carbonate solution were successively added, and the mixture was warmed at 42±1°, protected from light. After cooling in an ice-water bath, 0.12% solution of sodium dihydrogenphosphate was added to the mixture to make 5.0ml., and the developed color was measured at the absorption maximum of 460 mμ, with a reagent blank, which followed the Beer's rule in a concentration range of 0 to 100γ/ml. The appropriate concentration of sodium carbonate was 0.2% for I, 0.3% for III, and 0.35% for II and IV. The optimum heating time for the color development was 20 minutes for I, II, and III, and 25 minutes for IV.
Some active methylene compounds interfered with this coloration. Polyphenols, creatinine, indole, and androsterone gave a strong coloration with this reagent. Standard deviation of the method in a concentration of 50γ/ml. of the glycoside is given in Table I, compared with the J. P. method.
