When organic reagents with chelating functional groups was analysed by thin-layer chromatography, a better result can not be obtained because of the tailing. The investigation revealed that traces of metallic elements coexisted in adsorbents chelated with the reagents. In order to prevent this inconvenience, EDTA⋅2Na which is more than an amount consumed by the water-insoluble components of the adsorbent (Kieselgel G.) was added in advance to the chromato-plate for the better chromatography.
