Cellulose Thin-layer Chromatography of Monosaccharides.

Abstract

Separation of monosaccharides was attempted by thin-layer chromatography over cellulose, using various solvents and the results obtained are listed in Table I. The most effective procedure for general analysis of monosaccharides was found to be the combined use of a solvent system of butanol-pyridine-acetic acid-water (10 : 6 : 1 : 3), ethyl acetate-pyridine-water (10 : 3 : 2), or ethyl acetate-isopropanol-pyridine-water (7 : 3 : 2 : 2) with phenol-1% ammonia water (2 : 1).