Abstract
Binding of pentobarbital (sodium 5-ethyl-(1-methylbutyl)-barbiturate) with plasma of male and female rats, dogs, and monkeys was studied in vitro using the method of equilibrium dialysis in 1/15 M phosphate buffer (pH7.4). Rat plasma had the most extensive binding capacity, followed by dog and monkey plasma, and the plasma of female had more extensive capability than male in rat and dog, but it was reversed in monkey. The use of ether as an anaesthetic at the time of blood collection lowered the extent of binding.
