1968 年 88 巻 3 号 p. 254-258
Fluorescence reaction in acid medium was found between 4-hydroxyphthalaldehyde, 5-bromo-4-hydroxyphthalaldehyde, 4-cyanophthalaldehyde, 3, 4-diformylbenzoic acid, or 1, 2, 4, 5-tetraformylbenzene and p-anisidine, aniline, or sulfanilamide. The fluorescence reaction of these phthalaldehyde derivatives is due to the formation of 5, 6-substituted 2-phenylphthalimidine derivatives and it seemed possible to utilize this reaction for fluorescence analysis of aromatic primary amines. A highly sensitive fluorometric determination of sulfanilamides using various phthalaldehyde derivatives was established.