Rapid Separation of Metallic Ions and Barium¹⁴⁰-Lanthanum¹⁴⁰ by Thin-Layer Electrophoresis

Abstract

Some 34 common metallic ions were separated rapidly and identified by thin-layer electrophoresis on one piece (6.5×2 cm) of thin-layer Chromatosheet (Eastman Kodak Co., silicagel). Fundamental experiments were carried out at first using citric acid as the electrolytic solution. The relationship between the migration distance and migration time as well as the electric potential gradient, the concentration of citric acid, and pH of the electrolytic solution were examined. The electrophoresis was carried out for 5 min with 300 V/6.5 cm, using 0.1 M citric acid as the electrolytic solution. The samples were spotted at 1.5 cm from the chromatostrip end (anode side). Electrophoresis of ¹⁴⁰Ba-¹⁴⁰La was also carried out for 5, 7, and 10 min, and about 90% separation of ¹⁴⁰La was obtained by electrophoresis for 10 min.