Studies on the Aliphatic Amines in Human Urine. II. : On the Dinitrophenylation of Aliphatic Amines in Human Urine and Thin-Layer, Ion Exchange and Gas Chromatography of Their DNP-Derivatives

Abstract

The following investigations were carried out on : 1. The yield of dinitrophenyl derivatives of aliphatic amines in human urine and their extraction rate from the reaction mixture using various solvents. 2. The thin-layer chromatographic identification of aliphatic amines in urine of patients after dinitrophenylation. 3. The separatory estimation of ethanolamine, methylamine and dimethylamine in urine of patients by isolating their dinitrophenyl derivatives by ion exchange chromatography and reversed phase chromatography. 4. The gas chromatography of dinitrophenyl derivatives of aliphatic amines in human urine with SE-30 or OV-17 as liquid phase.