Studies on the Aliphatic Amines in Human Urine. III : On the Identification of Three New Aliphatic Amines in Human Urine

Abstract

The occurrence of three new aliphatic amines in human urine was definitely proved. A satisfactory analytical procedure was established by extracting the amine distillate derived from a large quantity of urine (100-200 ml) with chloroform and by removing a great part of ammonia and methylamine. Isobutylamine and n-butylamine were identified by analyzing the extract in the amino acid analyzer. Each fraction containing isobutylamine or n-butylamine was collected, dinitrophenylated and then submitted to gas chromatography to provide further identification of isobutylamine and n-butylamine as their DNP-derivatives. Moreover, n-hexylamine was evidently identified by thin-layer and gas chromatography.