Studies on the Inhibition of Enzymes by Addition of Isothiocyanates and Related Compounds. II : Effects of Isothiocyanates solubilized with Detergents on β-Amylase

Abstract

A previous work showed that barley β-amylase was inhibited by the addition of isothiocyanates in the enzyme reaction medium. As isothiocyanates were slightly soluble or insoluble in water, previous study was not performed under the condition of the solution competely. Using detergents for the solubilizing isothiocyanates in the present work, the effect of solubilized isothiocyanates on β-amylase was examined. It was found that β-amylase activity was not inhibited by the addition of isothiocyanates which were solubilized by Tween 20 or 80 in the medium.