1971 年 91 巻 8 号 p. 845-849
A low-temperature oxygen plasma generated in a high-frequency electromagnetic field under a low pressure was applied to plant tissues on a slide glass for removing organic matrices but perfectly preserving the shape and arrangement of calcium carbonate crystals in the specimens. The observation of ashed tissues of leaves of Ficus elastica ROXB. revealed details of cystolith deposited in the epidermis which seemed to be clustered crystals having optical properties of either isotropy or anisotropy. Calcium oxalate crystals were also observed along the vein in extremely high density. An ashed leaf of Himulus japonicus SIED. et ZUCC. was highly characterized by short hair of single cell which had semispherical base consisting of several cells encircling a cystolith ball inside. Justicia procumbens LINN. left slender cystolith in the ashed tissue irregularly arranging near the vein.