Abstract
A newly precoated plate for thin-layer chromatography (TLC) has been prepared, which is made from a mixture of Kieselguhr and fused glass powder. Because this sintered plate contains no organic binders such as starch, polyvinyl alcohol, polyethylene, etc., it is not charred under any drastic conditions, i.e. by heating after spraying corrosive reagents such as sulfuric acid, chromic acid mixture, trichloroacetic acid, antimony trichloride, etc. Thin-layer chromatographic separation of various organic compounds such as azo dyes, sugars, higher fatty acids, and polychlorinated biphenyls (PCB) was examined by using this new plate, and it was found that this plate gave good reproducibility of separation as reliable as the home-made plates prepared from the commercial Keiselguhr. This plate is mechanically stable, heat-stable, and acid-resistant. Moreover, it can be repeatedly used by soaking the chromatogram into cleaning solutions.
