Abstract
A fluorometric method for the determination of furfural was established based on the fact that furfural reacts with o-aminothiophenol to form 2-(2-furyl) benzothiazole which shows an intense fluorescence in a strong acid solution. Pentose was also found to react with o-aminothiophenol and the reaction mixture showed a reddish violet fluorescence from which 2-(2-furyl) benzothiazole was isolated and identified. Hexose also undergoes the same reaction. This fluorometric method is applicable for 0.1 -2.0μg/ml of furfural, 1.0-8.0μg/ml of pentose, and 1.0-10μg/ml of hexose.