Studies on Extraction, Separation and Estimation of Prostaglandins by Radioimmunoassay. II. Separation of Individual Prostaglandin by Thin-Layer Chromatography with Stepwise Development, and Determination of Prostaglandin E₂

Abstract

An examination was made for individual separation of prostaglandins (PGs) from living tissues or body fluid. Total PGs, extracted by the previously reported procedure¹⁾ were separated into PG E₁, E₂, F_1a, and F_2a by a stepwise development method applied to thin-layer chromatography on 5% (w/v) silver nitrate-sprayed silica gel HR plate after first development for separating to PGs E and PGs F. Rabbit PG E₂ antiserum, which has a high specificity to PG E₂, was also obtained. With the use of this antiserum, radioimmunoassay capable of measuring low levels of PG E₂ was developed. It was concluded from the results of PG levels measured by the individual separation method and the group separation method reported previously that each prostaglandin should be measured by the individual separation method.