Abstract
Non-steroidal anti-inflammatory drugs and α-tocopherol were tested in vitro for inhibition against hemolysis and lipid peroxidation induced by H2O2 in erythrocytes from healthy mongrel dogs. In concentration over the range of 10-6 to 4.5×10-4M, anti-inflammatory drugs inhibited both hemolysis and lipid peroxidation, while α-tocopherol had no effect. Against peroxidation of free unsaturated fatty acids caused by various means (H2O2, UV irradiation, ascorbic acid+Fe+2, and hemoglobin), antioxidants including α-tocopherol showed a marked inhibition, whereas almost all anti-inflammatory drugs had no effect. H2O2 was not destroyed directly by both antioxidants and anti-inflammatory drugs. Diphenyl-p-picrylhydrazyl, a model compound of free radical, was reduced powerfully with antioxidants but not with anti-inflammatory drugs.
