The theoretical equations for the membrane potential and the membrane permeability coefficient were derived on the basis of nonequilibrium thermodynamics. In this experiment, the system was constructed with two-cell chambers divided with a membrane and filled with solution of the same electrolyte but of different concentration. The Visking Cellotube was used as the membrane and the membrane potential and the membrane permeability coefficient were measured. The experimental temperature was 34°, and solutions of NaCl, KCl, LiCl, NH4Cl, and CaCl2 were used. By changing the concentration, the experimental membrane potential was in agreement with the theoretical values in a high concentration range. Also, charge density of the membrane was determined from the membrane potential and from the membrane permeability. These two values were in good agreement. Then P*, defined as the concentration gradient in the membrane, was calculated. The value of P* did not depend on the concentration of solution as much as did the membrane permeability coefficient P.