Abstract
Color reaction between hemin and pyridine in acetic acid was spectrochemically studied. Hemin sensitively reacts with pyridine in acetic acid to form pyridine hemichrome. A hemin solution in a mixture of pyridine and acetic acid (15 : 85, by vol.) shows a stable red orange color which gives an absorption maximum at 407 nm. A linear relationship was observed between the hemin concentration. in the range of 0-8.5 μg/ml and the absorbance at 410 nm in the solution. This color reaction can be used as a simple and rapid technique for the qualitative and quantitative analysis of hemin.
