Abstract
Determination of conjugated bile acids in bear gall by thin-layer chromatography was examined, using an apparatus equipped with a flame ionization detector. Neutral lipid-free methanolic extract from bear gall was spotted on a chromatographic rod, a fine rod of quartz coated with silica gel, and developed, Acids were separated quantitatively by means of multiple development with toluene : acetic acid : water (10 : 10 : 1) and hexane : diisopropyl ether : acetic acid (2 : 1 : 1). The calibration curve was constructed with the use of taurolithocholic acid as an internal standard.
