Abstract
Fractionation of serum lipid by means of the droplet countercurrent chromatography (D.C.C.) by the ascending method was investigated. D.C.C. column system was built 50 of or 100 glass tubes of 2.4×1200 mm, which were joined with each other with Teflon tubes of 0.8×1500 mm. The solvents, heptane : butanol : chloroform : methanol : 60% acetic acid (3 : 2 : 2 : 3 : 5, v/v) and heptane : benzene : chloroform : ethanol : 60% acetic acid (2 : 3 : 1 : 4 : 3, v/v) were found to be suitable in consideration of their physical properties, such as density, distribution coefficient for lipid, and passing rate of droplet in columns of stationary phase. By this method, human serum lipid was able to be separated into three fractions of neutral fat, fatty acid, and phospholipid, and the separated phospholipid was found to be a mixture of cephalin, lecithin, and sphingomyelin by silica gel thin-layer chromatography.
