Abstract
A rapid, simple, and accurate high-speed liquid chromatographic procedure was established for the quantitative determination of glycyrrhizin in pharmaceutical preparations including Glycyrrhiza radix. Glycyrrhizin, extracted from simple pharmaceutical preparations, was determined under the same conditions as previously. Glycyrrhizin in compound pharmaceutical preparations containing multiple components was separated on Permaphase AAX column (1 m×3 mm, internal diam.) with a mobile phase of 50% ethanol in phosphate buffer (pH 5.2)-0.005 M NaClO4 solution at 45°. In most of preparations, good recovery was obtained, but in a few, sufficient recovery was not obtained because of incomplete extraction.
