1977 年 97 巻 1 号 p. 106-108
A rapid estimation method utilizing high-speed liquid chromatography is described for determination of ascorbic acid 2-sulfate (AsS). Separation can be achieved within 6 min employing a 1-m column packed with Zipax SAX using 0.0005 M Na2B4O7-0.05 M NaClO4 (93 : 7, v/v) (pH 7.0) solution as an eluant in a Du Pont LC 830 liquid chromatograph. Typical chromatogram obtained by this method are shown. Precision in this method was found to be less than ±2.0% with the relation Y=0.31X+0.07 and a relative coefficient of 0.999. The limit of detection was 40 ng. AsS from human urine was examined by this analytical method. The peak component was identified by ultraviolet absorption spectra and thin-layer chromatography as AsS.