A New Fluorometric Analysis of Dulcin Using Sodium Nitrite. V. Enhancement of Fluorescence Intensity by Addition of Acetaldehyde

Abstract

By the addition of acetaldehyde into the reaction system, the fluorescence determination of dulcin³⁾ was improved by enhancing the fluorescence intensity five times. By the use of a similar reaction system, a new qualitative test of dulcin by a thin-layer chromatographic method was developed, in which the detection limit was 20 times lower than that of the conventional one. In addition, aniline derivatives, especially p-anisidine and p-phenetidine, were detected with a high sensitivity by the present method.