1977 年 97 巻 4 号 p. 455-458
A rapid estimation procedure utilizing high-speed liquid chromatography is described for the determination of acrinol (6, 9-diamino-2-ethoxyacridine lactate monohydrate) in pharmaceutical preparations. Separation can be achieved within 7 min at 45° (flow rate, 2.1 ml/min) employing a 1 m×2.1 mm id. column packed with Zipax SAX using 0.05M NaClO4 (pH 3.3) solution as an eluant in a Du Pont 830 liquid chromatograph. Acrinol in a pharmaceutical preparation was extracted with 20% (v/v) AcOH and injected into a column. Accuracy in this method was found to be less than ±1.5% with the relation Y=0.251x+0.912 (X=quantity of acrinol in ng, Y=peak height of acrinol in mm) and a relative coefficient of 0.999.