抄録
As reported previously p-dimethylaminocinnamaldehyde reacts with 4-aminoantipyrine to produce an intense color, which can be used as an analytical reagent. A similar color reaction and colorimetric determination were examined with other aminopyrinerelated compounds, 4-formylaminoantipyrine (I), 4-monomethylaminoantipyrine (II), and 4-acetylaminoantipyrine (III), and optimum reaction conditions for color development were examined. The relationship between absorbance and concentration of I, II, and III showed linear plots in the range of 0.6-2.5 μg/ml of I and II, and 6.25-25.0 μg/ml of III. Interference of water decreases the absorbance of aminopyrine-related compounds and indicated the necessity for removal of water. Thin-layer chromatography was applied for the detection of various aminopyrine-related compounds, using p-dimethylamino-cinnamaldehyde, and it was found possible to detect these compounds distinctly.
