Simultaneous Determination of Glycyrrhizin and Glycyrrhetic Acid in Plasma by High Speed Liquid Chromatography

Abstract

Simultaneous determination of glycyrrhizin (G) and glycyrrhetic acid (GA) in plasma was established by high-speed liquid chromatography. G and GA were extracted from plasma with 0.25 N NH₄OH·85% EtOH. The extracted sample containing indomethacin as an internal standard was analysed on a reverse-phase column (SC-02-250) with gradient elution from water to 0.5% acetic acid-90% acetonitrile.