和漢薬作用に関する薬学的基礎研究(第13報)牛胆汁中の平滑筋収縮性物質の薬理学的探索
1978 年 98 巻 9 号 p. 1139-1144
詳細
1978 年 98 巻 9 号 p. 1139-1144
The fraction of smooth muscle-contracting substances (SMC) extracted from dry ox bile was examined using isolated guinea pig ileum. The dialyzable SMC fraction was soluble in water and ethanol, and reacted with both ninhydrin and Dragendorff's reagents. The contracting activity in products of the SMC fraction hydrolyzed by crude pseudocholinesterase or hydrochloric acid decreased to less than 1/10 or to less than 1/100, respectively, though did not decrease by either α-chymotrypsin or trypsin. Active choline esters and ninhydrin-positive substances were obtained from the SMC fraction, using ion-exchange column chromatography on Dowex 50W-X2.
