High-speed Liquid Chromatographic Analysis of Drugs. III. Rapid Estimation of Arbutin in Crude Drugs

Abstract

A rapid determination of arbutin in crude drugs (uva ursi, cowberry, and uva ursi extract) by the use of high-speed liquid chromatography is described. Arbutin is separated by the 25 cm column of Zorbax CN, using liquid chromatograph of Shimadzu-Dupont Model LC-2, with water as the desorption solution, and is completed within 15 min. Arbutin in the crude drugs is extracted with water and the aqueous extract is injected into the column. Precision of determination is ±2% and detection limit is a few nanograms of arbutin.