高速液体クロマトグラフ法による医薬品の分析(第5報)シャクヤク中のペオニフロリンの迅速定量
1979 年 99 巻 8 号 p. 858-861
詳細
1979 年 99 巻 8 号 p. 858-861
A method for the rapid estimation of paeoniflorin in crude drugs of paeony root was established by the use of a high-speed liquid chromatography. Paeoniflorin is separated by a 25 cm column of Zorbax CN, using liquid chromatograph of Shimadzu-Du Pont Model LC-2, with 0.1 M HClO4 (pH 3.5)-CH3CN (9 : 1, v/v) as the desorption solution, and the separation is completed within 10 min. Paeoniflorin is the crude drugs is extracted with water and the aqueous extract is injected into the column. The content of paeoniflorin was calculated from the calibration curve previously prepared using a standard. Precision of the determination is about±1% and detection limit is 15 ng (S/N ratio, 3). This method is considered to be useful for the evaluation of paeony root.
