Use of a Single-Tube Nested Real-Time PCR Assay to Facilitate the Early Diagnosis of Acute Q Fever

Min-Yi Hou; Min-Nan Hung; Po-Shan Lin; Yung-Chung Wang; Chien-Chou Lin; Pei-Yun Shu; Wen-Yi Shih; Ho-Sheng Wu; Li-Jen Lin

doi:10.7883/yoken.64.161

Short Communications

Use of a Single-Tube Nested Real-Time PCR Assay to Facilitate the Early Diagnosis of Acute Q Fever

Min-Yi Hou, Min-Nan Hung, Po-Shan Lin, Yung-Chung Wang, Chien-Chou Lin, Pei-Yun Shu, Wen-Yi Shih, Ho-Sheng Wu, Li-Jen Lin

著者情報

Min-Yi Hou
Laboratory, Research and Diagnostic Center, Centers for Disease Control, Taiwan
Min-Nan Hung
The Fifth Branch, Department of Health, Centers for Disease Control, Taiwan
Po-Shan Lin
Laboratory, Research and Diagnostic Center, Centers for Disease Control, Taiwan
Yung-Chung Wang
Laboratory, Research and Diagnostic Center, Centers for Disease Control, Taiwan
Chien-Chou Lin
Laboratory, Research and Diagnostic Center, Centers for Disease Control, Taiwan
Pei-Yun Shu
Laboratory, Research and Diagnostic Center, Centers for Disease Control, Taiwan
Wen-Yi Shih
Department of Health, Centers for Disease Control, Taiwan
Ho-Sheng Wu
Laboratory, Research and Diagnostic Center, Centers for Disease Control, Taiwan
Li-Jen Lin
The Fifth Branch, Department of Health, Centers for Disease Control, Taiwan

ジャーナルフリー

2011 年 64 巻 2 号 p. 161-162

DOI https://doi.org/10.7883/yoken.64.161

詳細

抄録

We have developed a single-tube nested real-time PCR (STN-RT PCR) assay using the repetitive, transposon-like element IS1111 as the DNA target to facilitate early diagnosis of acute Q fever. The use of our proposed diagnostic procedures, including IgM detection by serology and the STN-RT PCR assay, significantly increased the diagnostic sensitivity for Q fever to 78%, compared to 29% when serology alone was used for subjects providing mainly acute-phase blood samples.

責任著者(Corresponding author)

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