抄録
To determine the resistance patterns of uropathogenic Escherichia coli (UPEC) isolates and to investigate the frequency of several virulence genes, fimH, papA, hlyD, cnf-1, sitA and tsh, among different phylogenetic groups of UPEC isolates. A total of 85 E. coli isolates were recovered from urine samples from outpatients with clinical diagnosis of uncomplicated urinary tract infections. Molecular approach and antimicrobial resistance pattern were done by PCR and disc diffusion method. The frequency of virulence factor genes detected by PCR was: fimH (34.1%), papA (9.4%), hlyD (21.2%), cnf-1 (3.5%), sitA (15.3%) and tsh (27.1%). The results revealed that isolates were resistant to SXT (74.1%), CTX (68.2%) and AMC (94.1%) and relatively less resistance to N (56.5%). According to the results, larger investigations are suggested to be done in future to exactly determine whether SXT, CTX and AMC are appropriate antibiotics for treatment of UPEC infections in southern Iran or not. Although these results have demonstrated that fimH is the most frequent virulence genes among UPEC, but high prevalence of isolates which do not encoded fimH (75.9%) and also relatively low frequency of isolates carrying other virulence genes need further investigations to clarify the role of other potential virulence factors in pathogenesis of these isolates.
