Development of Genetic Diagnostic Methods for Novel Coronavirus 2019 (nCoV-2019) in Japan

Kazuya Shirato; Naganori Nao; Harutaka Katano; Ikuyo Takayama; Shinji Saito; Fumihiro Kato; Hiroshi Katoh; Masafumi Sakata; Yuichiro Nakatsu; Yoshio Mori; Tsutomu Kageyama; Shutoku Matsuyama; Makoto Takeda

doi:10.7883/yoken.JJID.2020.061

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引用する場合も本公開記事を引用してください。

Development of Genetic Diagnostic Methods for Novel Coronavirus 2019 (nCoV-2019) in Japan

Kazuya Shirato, Naganori Nao, Harutaka Katano, Ikuyo Takayama, Shinji Saito, Fumihiro Kato, Hiroshi Katoh, Masafumi Sakata, Yuichiro Nakatsu, Yoshio Mori, Tsutomu Kageyama, Shutoku Matsuyama, Makoto Takeda

著者情報

Kazuya Shirato
Department of Virology III, National Institute of Infectious Disease
Naganori Nao
Department of Virology III, National Institute of Infectious Disease
Harutaka Katano
Department of Pathology, National Institute of Infectious Disease
Ikuyo Takayama
Influenza Virus Research Center, National Institute of Infectious Disease
Shinji Saito
Influenza Virus Research Center, National Institute of Infectious Disease
Fumihiro Kato
Department of Virology III, National Institute of Infectious Disease
Hiroshi Katoh
Department of Virology III, National Institute of Infectious Disease
Masafumi Sakata
Department of Virology III, National Institute of Infectious Disease
Yuichiro Nakatsu
Department of Virology III, National Institute of Infectious Disease
Yoshio Mori
Department of Virology III, National Institute of Infectious Disease
Tsutomu Kageyama
Influenza Virus Research Center, National Institute of Infectious Disease
Shutoku Matsuyama
Department of Virology III, National Institute of Infectious Disease
Makoto Takeda
Department of Virology III, National Institute of Infectious Disease

キーワード: novel coronavirus 2019, nCoV-2019, nested RT–PCR, real-time RT–PCR

ジャーナルフリー早期公開

論文ID: JJID.2020.061

DOI https://doi.org/10.7883/yoken.JJID.2020.061

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2020), No. 4

詳細

抄録

At the end of 2019, pneumonia caused by novel coronavirus 2019 (nCoV) emerged in Wuhan city, China. Many airline travelers moved between Wuhan and Japan at that time, suggesting that Japan is at high risk of invasion by the virus. Diagnostic systems for 2019-nCoV were developed with urgency. Two nested RT–PCR assays and two real-time RT–PCR assays were adapted to local Japanese conditions. As of 8 February 2020, the assays developed have successfully detected 25 positive cases of infection in Japan.

責任著者(Corresponding author)

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