A number of antibiotics have been reported to have the antiamebic effect. Among them are anisomycin, chlortetracycline, oxytetracycline, erythromycin, cycloheximide, and prodigiosin, most of which have been tested in the treatment of amebiasis and proved to be more or less effective. Several other antibiotics such as fumagillin, trichomycin, eurocidin and candimycin which are effective for eumycetes or malignant tumors have been also found to have antiamebic activities.
Most of the antibiotics cited above, however, have the antibacterial effects. The growth of Entamoeba histolytica in culture media is much influenced by the associated bacterial flora. It has been demonstrated by many investigators that some kinds of bacteria well support the growth of amebae while others do not. The ameba cannot grow in culture media without the accompanying growth of some symbiotic bacterial species. Therefore, it is very difficult to test in vitro the antiamebic effect of some antibiotics which have the bacteriostatic activities. They may stop the bacterial growth and only secondarily inhibit the growth of amebae.
In the present study, the antibiotics which do not have antibacterial effect were selected for screening test. All of the culture filtrates of streptomycetes tested were prepared in the Laboratory of Antibiotics in our Institute under the direction of Dr. Hata and were sent to our Parasitology Laboratory. By the screening tests of the culture filtrates of more than 300 strains of streptomycetes, a number of filtrates were found to have the amebicidal activities in vitro. Extract from one of these effective filtrates, which was named Protomycin was studied extensively on its amebicidal effects in vitro and in vivo. Results of the experimental works on this substance are described in this report.
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