Shiitake mushrooms (Lentinula edodes (Berk.) Pegler) were cultivated using sawdust medium containing test water to which heavy metals and other metal, namely Cd, Pb, As, and Hg, were added at concentrations higher than the standard concentrations in drinking water. Further, the concentrations of these metals in the fruit-bodies (pilei) were assessed. The metal concentrations in the test water were adjusted as 1, 2, 4, 8, and 16 times higher than the standard concentrations in drinking water. We observed that the concentrations of 3 metals, except Pb, in the pilei tended to be higher with increase in metal concentrations in the test water. Even when using test water with metal concentrations that were equal to the standard concentrations in drinking water, the metal concentrations in the pilei were higher than those in the test water. Thus, we considered the accumulation of metals from the sawdust and nutrition in the medium, in the fruit-bodies. The Cd concentrations in pilei at Cd test water concentrations of 8 and 16 times were, respectively, about 60 and 70 times higher than the standard concentrations in drinking water. This indicated that Cd concentrated in the fruit-bodies. Further, As concentrations of 1-8 times resulted in the pilei showing As concentrations higher than that in the test water and basis substrate, which suggested that As also concentrated in the fruit-bodies. Based on these results, the necessity of establishing the heavy metal and other metal standard in water added to the culture medium was discussed for confirming food hygiene of Shiitake mushroom.
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