The concentration of major ions (mM/l) in haemolymph of the whip scorpion,
Thelyphonus indicus STOLICZKA, were found to be Na
+=222.6, K
+=14.24, Ca
2+=12.50, Mg
2+=7.52 and Cl
-=235.16. The pH of haemolymph was found to be 7.60. Based on these values the saline formula was established as follows: NaCl =13.00g/l, KCl=1.06g/l, CaCl
2=1.38g/l, MgCl
2=1.52g/l. The pH was adjusted to 7.60 with 0.2M Tris-HCl buffer. The suitability of the prepared saline for neurophysiological studies has been tested.
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