Deep seawater (DSW) inhibits the calcification of normal human dermal fibroblasts (NHDF) caused by UVA irradiation. Previously, we reported that the expression of the alkaline phosphatase (ALP) activity of NHDF was induced on the calcification of NHDF caused by UVA irradiation and that DSW suppressed the expression of the ALP activity induced by UVA irradiation. The purpose of this study is to research the relationship between the expression of the calcification and the ALP activity of NHDF induced by UVA irradiation using DSW and two kinds of well known ALP inhibitors. In this study, electrodialyzed deep seawater (EDW) was prepared, because its evaluation was better than DSW in the irritation test using SIRC cells from rabbit cornea. First, the effect of EDW on the calcification of NHDF caused by UVA irradiation was investigated. Next, the effect of EDW on ALP activity was investigated. Furthermore, effects of etidoronic acid (ETA) and homo arginine (HA), which were well-known as ALP inhibitors, were investigated on the calcification of NHDF caused by UVA irradiation. As a result, it was suggested that the suppressive effect of these inhibitors on the calcification of NHDF caused by UVA irradiation was limited. So, the effect of the use of both EDW and an ALP inhibitor was investigated on the calcification caused by UVA irradiation at the last step in this study. As a result, the addition of both EDW and ALP inhibitors remarkably inhibited the calcification of NHDF caused by UVA irradiation. It was suggested that the addition of both EDW and ALP inhibitors has a synergistic effect on inhibition of the calcification, because this inhibitory effect on the calcification was not able to be explained by the suppressive activity on ALP activity only.

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In 2013, Okinawa prefecture had launched the ocean thermal energy conversion (OTEC) demonstration plant and been operated for about six years in Okinawa prefectural government deep seawater research center (OPDSRC), Japan. The variety of high-develop business utilizing the deep ocean water (DOW) advances in Kumejima island by applying in the aquaculture, agriculture, bath, drinking water and cosmetic products. And, eventually, the DOW industry becomes the biggest community in terms of economical impact in the island. Furthermore, an expected increase of DOW intake capacity for expansion of DOW usage and for introduction of OTEC. In general, the function of DOW is the annual low temperature stability, the clearness, and inorganic nutrient-rich; however, the vertical profile data of the nutrient is limited in the coast of Kumejima. In this paper, therefore, the authors measured the sea water temperature, salinity, and DO by CTD, and sampled in each depth in four locations of the Kumejima east side. The sampled seawater were analyzed the concentration of inorganic nutrients. This research clarifies the vertical profiles of the basic data that is necessary for the application and clarification of the potential of OTEC and DOW multiple utilization.

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Various bioactive compounds have been discovered from actinomycetes; however, it is difficult to obtain a new compound in recent years. Therefore, a new group of actinomycetes is required which has a different potential for secondary metabolite production. In this study, we report the isolation and metabolites of actinomycetes in Toyama Bay. The results of genus-level identification, 110 actinomycetes isolates were identified as Streptomyces (88 strains), Micromonospora (19strains), Actinomadura (2 strains), and Nocardiopsis (1 strain). The metabolite analysis by the HPLC-UV spectra database showed that 41％ of strains produced unknown UV compounds. The selected strain produced 5 new compounds. These results indicated that deep-sea water is a useful isolation source of actinomycetes for drug discovery.

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The intake of magnesium (Mg) which is one of the essential nutrients for human health has been decreasing in Japan since the national health and nutrition survey started in 1946. Therefore, efficient methods of Mg intake have been expected. Bread is the focus of attention as a food for efficient Mg intake because it has been become one of the daily foods in Japan. In this study, we investigated the effect of water, which was used as the second largest ingredient following wheat flour as a material for bread making. Deep seawater (DSW) which contained rich Mg was used for bread making as the test water and tap water (TW) was used as a control. Bread making was carried out using a commercially available home bakery machine in this study. The amount of Mg in the test bread, the increment of which is the most important object in this study, and the amounts of other major minerals (Na, K, Ca) included in them were measured. In addition, the texture and physical characteristics of the test bread were evaluated. As a result, it was elucidated that the test bread was 5.3 times larger than the control bread in the amount of Mg. On the other hand, there were few differences in the texture and physical characteristics between the test bread and the control bread, which provided useful results in this study. Furthermore, it was elucidated that the test bread was higher than the control bread in a moisture retention capacity. From these results, it was suggested that DSW could be a promising ingredient in bread making for ingesting Mg efficiently.

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