The concentrations of acid volatile sulfides (AVS) in three different size fractions (< 10 nm, 10-200 nm, > 200 nm) at lake Teganuma was measured using nano-size filtration method and a series of acid addition, Helium purge, and GC-FPD analysis. Fresh water sampling was obtained from four sites and filtration using 30 nm or 0.2 µm pore size filters for size fractionation was immediately made on site. The concentrations of AVS for unfiltered fractions were in the range of 0.6-1.4 nmol/kg in this study, and the highest values of AVS were observed in the lotus field station. For the relative concentration ratios of size fractionated AVS for three size fractions of < 30 nm, 30-200 nm and > 200 nm, it is almost same ratios for the all stations, except for the lotus station, and ~80% of metal sulfides fractions were existed as size fractions of < 10 nm, and there were only 10% or so for both 30-200 nm and > 200 nm size fractions with respect to the unfiltered fraction. On the other hand, in the lotus station, the contribution of < 30 nm fraction was contributed only ~5% and ~80% of metal sulfides fractions were significantly existed as size fractions of 30-200 nm, although the total AVS concentration of the lotus station is almost the same level as the other stations.
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